N= 29 Rats Wistar
Poids moyen : 421g

Provenance des animaux : Biotério Central of Campus of Ribeirão Preto of São Paulo University
Maintenance : Experimental Pathology Lab of Pathology Department on Medicine School of Ribeirão Preto – USP
Conservation des Animaux: boites de polypropylène 
Alimentation des Animaux : dète basique du laboratoire et eau
Conditions d éclairage: lumière naturelle/cycles dans le noir sous controle de conditions basiques
Experimentateurs : A.Tenenbaum,R.Mené,Kim,M.Tiziani

Les rats furent euthanasiés dans des chambres à CO2 .

• Incision de la peau avec une lame de bistouri nº 23
• Recherche and isolation du muscle prétibial en utilisant des ciseaux de Metzenbaum
• Section des tendons au moyen de ciseaux .
• Fixation de segments de muscles sur des tronçons de chêne-liège.
• Fixation des préparations utilisant une solution au formaldéhyde - 10% tamponée avec 50% de pure paraffine pour une étude au microscope optique conventionnel. 
• Staining Sections as routine histological process.
• Microtome utilisé : Leika RM 2155
• Coloration des tranches de muscle au moyen de  

-hematoxiline-éosine
-Trichrome de Masson
-et picro-sirius rouge.

• Un Examen histologique fu réalisé au moyen d un microscope conventionnel (Olympus BH-2).
• Observation sous  lampe polarisée
• Documentation photographique was realized with a Leika DMR microscope joined to a digital camera Leika CD 300F and compatible PC.

• Animals were divided in 6 boxes, so that boxes from number 1 to 5 had 5 rats and box number 6 had 4 rats 
• All animals were submitted to a walking evaluation by footprint impression on special paper , and previous immersion of back legs on a soap solution. 
• Box 1 had the control group, that received the application of 0,1 ml saline solution ( NaCl 0,9%) on the right pretibial muscle. 

• The mice of boxes 2 to 5 received 0,1 ml of Endopeels Original Main Product on the same muscle as control group.

• Box 6 was constituted of 4 rats, and each one received 0,5 ml of Endopeels original main product injected on the subcutaneous layer. 

• All the animals were submitted to walking evaluation before and after their respective injections.

1 month after Endopeel Injection 0.1ml in the right pretibial muscle.
What is seen in black on the pictures is not a necrosis like could imagine some scientifics !
In fact, 4 conclusions have to be taken in consideration

• an artefact of coloration
• an absence of necrosis
• an apoptosis
• a bioregenerative process 

3 months (D90)after Endopeel Injection 0.1ml in the right pretibial muscle.

7 months (D210)after Endopeel IM Injection 0.1ml in the right pretibial muscle.

Complete Restitutio ad integrum after 7 months

0.5 ml ( 5x 0.1ml)  Endopeel SC Injection in the right subcutaneous pretibial area.